- High-fidelity combined with improved specificity of hotstart
- Convenient PCR setup at room temperature
- No unwanted secondary extensions, reduced background
- Very high specificity and PCR sensitivity
innuTaq HOT-A DNA Polymerase
- Product Information
ProductinfoinnuTaq HOT-A DNA Polymerase provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5` → 3` direction in the presence of magnesium. It also possesses a 5` → 3` polymerization- dependent exonuclease replacement activity but lacks a 3` → 5` exonuclease activity.
innuTaq HOT-A DNA Polymerase requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
Packages Weight incl. packaging (may differ) 0.0 g Package height (may differ) 0.0 m Width of package (may differ) 0.0 m Length of package (may differ) 0.0 m
Amplification of a 1 kb DNA fragment using innuTaq HOT-A DNA Polymerase
Lane 1: 1 kb DNA ladder
Lane 2 – 4: innuTaq HOT-A DNA Polymerase
- Items delivered
Storage conditions / Stability / Delivery
Store at –20 °C, avoid frequent thawing and freezing.