1. Analytik Jena
  2. Reagents
  3. PCR: polymerases and master mixes
  4. innuTaq HOT-A DNA Polymerase
innuTaq HOT-A DNA Polymerase product photo
innuTaq HOT-A DNA Polymerase product photo
  • innuTaq HOT-A DNA Polymerase product photo Front View 2XS

innuTaq HOT-A DNA Polymerase

Ordernumber: 845-EZ-3000050
Manufacturer: AJ Innuscreen GmbH
€ 20.00 plus VAT - free shipping

In Stock

Product Information

Features

  • High-fidelity combined with improved specificity of hotstart
  • ƒƒConvenient PCR setup at room temperature
  • ƒƒNo unwanted secondary extensions, reduced background
  • ƒƒVery high specificity and PCR sensitivity

Productinfo

innuTaq HOT-A DNA Polymerase provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5` → 3` direction in the presence of magnesium. It also possesses a 5` → 3` polymerization- dependent exonuclease replacement activity but lacks a 3` → 5` exonuclease activity.

innuTaq HOT-A DNA Polymerase requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
Specifications
Packages  
Weight incl. packaging (may differ)0.0 g
Package height (may differ)0.0 m
Width of package (may differ)0.0 m
Length of package (may differ)0.0 m
Applications
Amplification of a 1 kb DNA fragment using innuTaq HOT-A DNA Polymerase
Lane 1: 1 kb DNA ladder
Lane 2 – 4: innuTaq HOT-A DNA Polymerase
Items delivered

Storage conditions / Stability / Delivery

Store at –20 °C, avoid frequent thawing and freezing.