innuSPEED Tissue RNA Kit product photo
innuSPEED Tissue RNA Kit product photo
  • innuSPEED Tissue RNA Kit product photo Front View 2XS

innuSPEED Tissue RNA Kit

Ordernumber: 845-KS-2540010
Manufacturer: AJ Innuscreen GmbH
€ 67.00 plus VAT - free shipping

In Stock

Product Information

Features

  • Isolation of total cellular RNA from a variety of tissues
  • Kit contains Lysis Tubes with beads for homogenization
  • Optimized for using the SpeedMill
  • ƒƒMay also be used with other homogenizers
  • Improved extraction without the use of toxic β-mercaptoethanol

Productinfo

The innuSPEED Tissue RNA Kit allows users to isolate total RNA from a variety of tissue samples quickly and efficiently through the use of a homogenizer. A SpeedMill (or other homogenizer) is used as an effective means of digesting the starting material within the Lysis Tubes contained in the kit. Lysis is performed without the use of highly toxic β-mercaptoethanol, after which the genomic DNA is removed through an initial Spin Filter column. The RNA is then bound to a second Spin Filter membrane, washed and finally eluted in 30 – 80 µl of RNase-free water.

Detail Description

1. Homogenize and lyse the tissue sample
2. Use an initial Spin Filter column to selectively remove the gDNA
3. Bind the RNA to a second Spin Filter
4. Wash the bound RNA
5. Perform final elution
Specifications
Extraction  
A260 : A2801.7 - 2.0
Starting material for extractionTissue samples of up to 20 mg
ƒƒBioptates
Ø YieldDepends on the type and quantity of the starting material
ƒƒUp to 100 µg
Binding capacityColumn binding capacity: > 100 µg RNA
Duration of extractionHomogenization and lysis: approx. 20 minutes
ƒƒPurification: approx. 11 minutes
HandlingNA
Lysis Tubes  
Tube sizeNA
Packages  
Weight incl. packaging (may differ)0.3 kg
Package height (may differ)10.0 cm
Width of package (may differ)19.0 cm
Length of package (may differ)10.0 cm
Applications
Extraction of total RNA from 20 mg tissue samples followed by visualization on a denaturing formaldehyde gel.

Lane 1 – 3: Total RNA isolated from 20 mg tissue samples
Items delivered

Scope of delivery / Kit components

Lysis Tubes P, Lysis Solution, Washing Solutions, RNase-free water, Spin Filter D (blue), Spin Filter R (purple), Receiver Tubes, Elution Tubes, user manual

Storage conditions / Stability / Delivery

The innuSPEED Tissue RNA Kit will remain stable for at least 12 months if stored in a dry place at room temperature (14 °C to 25 °C).