innuSPEED Soil DNA Kit product photo
innuSPEED Soil DNA Kit product photo
  • innuSPEED Soil DNA Kit product photo Front View 2XS

innuSPEED Soil DNA Kit

Ordernumber: 845-KS-1580010
Manufacturer: AJ Innuscreen GmbH
€ 50.00 plus VAT - free shipping

In Stock

Product Information

Features

  • Isolation of microbial DNA from soil samples
  • ƒƒIncludes homogenization (SpeedMill, for instance) using Lysis Tubes specific to the application
  • ƒƒHigh-quality DNA free of any inhibitors (such as humic acids)
  • Suitable for use with homogenizers (such as the SpeedMill or others)

Productinfo

The innuSPEED Soil DNA Kit is a tool for extracting microbial DNA from soil samples. The extraction combines rapid homogenization of extremely complex starting material with efficient digestion of microbial cell walls in specially developed Lysis Tubes (using a SpeedMill or other homogenizer). After breaking down the material mechanically followed by a thermal lysis step, the DNA is selectively bound to the surface of a Spin Filter membrane. A novel wash buffer efficiently removes inhibitors such as humic acids. Finally, DNA is desorbed from the membrane through the addition of a low-salt buffer.

Detail Description

1. Homogenize and lyse the soil sample
2. Perform preliminary and final binding steps to bind DNA to the Spin Filter
3. Perform preliminary and final washing steps on the bound DNA
4. Perform preliminary and final DNA elution steps
Specifications
Extraction  
A260 : A2801.7 - 2.0
Starting material for extraction

Soil samples (100 – 250 mg)

Ø YieldDepends on the type and quantity of microorganisms in the soil
ƒƒUp to 30 µg
Binding capacityColumn binding capacity: > 30 µg DNA
Duration of extractionHomogenization and lysis: 30 minutes
ƒƒPurification: approx. 16 minutes
HandlingNA
Lysis Tubes  
Tube sizeNA
Packages  
Weight incl. packaging (may differ)0.4 kg
Package height (may differ)10.0 cm
Width of package (may differ)19.0 cm
Length of package (may differ)10.0 cm
Applications

Isolation (double determination) of microbial DNA from 6 different soil samples (Amazon rain forest). The isolated DNA was first applied to an 0.8 % TAE agarose gel and then introduced into a PCR specific to the microorganisms in question.

Microbial DNA after purification
PCR product specific to microorganisms
Lane 1: DNA ladder
lane 2 – 3: soil sample I
lane 4 – 5: Soil sample II
lane 6 – 7: Soil sample III
lane 8 – 9: soil sample IV
lane 10 – 11: Soil sample V
lane 12 – 13: soil sample VI
Items delivered

Scope of delivery / Kit components

Lysis Tubes B, Lysis Solution, Binding Solutions, Washing Solutions, Elution Buffer, Spin Filter (vanilla), Receiver Tubes, Elution Tubes, user manual

Storage conditions / Stability / Delivery

The innuSPEED Soil DNA Kit will remain stable for at least 12 months if stored in a dry place at room temperature (14 °C to 25 °C).