- Efficient extraction of total plant RNA from a wide variety of plant materials
- Optimized Lysis Tubes with beads guarantee optimum homogenization results when using the SpeedMill or other homogenizers
- Optimum sample digestion generates extremely high yields
- No need for toxic b-mercaptoethanol
- Product Information
ProductinfoThe innuSPEED Plant RNA Kit allows researchers to extract total cellular RNA from an extremely wide variety of fresh or frozen plant tissue (such as leaves, stems, roots, flowers, etc.) The Lysis Tubes in the kit contain beads that are specially tailored to plant tissue and that optimize the ability of a homogenizer (such as the SpeedMill from Analytik Jena) to break down the material. The genomic DNA is then selectively removed by binding it to an initial Spin Filter column. The total plant RNA is then bound to the filter membrane on a second column, washed and finally eluted in 30 – 80 µl of RNasefree water. Rapidly accelerated beads are used to solubilize plant cell walls efficiently, which, in turn, maximizes RNA yields.
1. Homogenization and Lysis of starting material 2. Selective removal of DNA by a first Spin Filter 3. Binding of RNA to a second Spin Filter 4. Washing of bound RNA 5. Final Elution
Daten laut MPG CE IVD No Distributor Analytik Jena AG Manufacturer AJ Innuscreen GmbH Extraction A260 : A280 1.8 - 2.1 Starting material for extraction Up to 50 mg Ø Yield In dependance on the starting material up to 100 µg Binding capacity > 100 µg RNA Elution volume 30 - 80 µl RNase-free water Duration of extraction Homogenization and lysis: approx. 15 min, Extraction: approx: 12 min Basis Filter membrane Handling Manual processing of Spin Filter Columns Storage / Stability of extraction kit 18 month at room temperature (15 °C to 30 °C) Packages Weight incl. packaging (may differ) 300.0 g Package height (may differ) 0.1 m Width of package (may differ) 0.19 m Length of package (may differ) 0.1 m
Extraction of Plant RNA
The innuPREP Plant RNA Kit was used to extract total cellular RNA from 50 mg leaf material and compared to the innuSPEED Plant RNA Kit. Subsequent analysis of the quality of the isolated nucleic acids was performed on an Agilent Bioanalyzer. Data indicate total plant RNA with intact ribosomal RNA, free of genomic DNA contamination and with no sign of degradation.
Sample 1: innuSPEED Plant RNA Kit with RIN 9.8; Sample 2: innuPREP Plant RNA Kit with RIN 9,.
- Items delivered
Scope of delivery / Kit componentsLysis Tubes P, Lysis Solution, Washing Solutions, RNase-free water, Spin Filter, Receiver Tubes, Elution Tubes, user Manual
Storage conditions / Stability / DeliveryThe innuSPEED Plant RNA Kit will remain stable for at least 18 months if stored in a dry place at room temperature (14 °C to 25 °C).