innuSPEED Plant RNA Kit product photo
innuSPEED Plant RNA Kit product photo
  • innuSPEED Plant RNA Kit product photo Front View 2XS

innuSPEED Plant RNA Kit

Highly efficient Isolation of plant RNA

Ordernumber: 845-KS-2560010
Manufacturer: AJ Innuscreen GmbH
€ 66.00 plus VAT - free shipping

Out of Stock

Product Information

Features

  • Efficient extraction of total plant RNA from a wide variety  of plant materials
  • Optimized Lysis Tubes with beads guarantee optimum homogenization results when using the SpeedMill or  other homogenizers
  • Optimum sample digestion generates extremely  high yields  ƒ
  • No need for toxic b-mercaptoethanol

Productinfo

The innuSPEED Plant RNA Kit allows researchers to extract total cellular RNA from an extremely wide variety of fresh or frozen plant tissue (such as leaves, stems, roots, flowers, etc.) The Lysis Tubes in the kit contain beads that are specially tailored to plant tissue and that optimize the ability of a homogenizer (such as the SpeedMill from Analytik Jena) to break down the material. The genomic DNA is then selectively removed by binding it to an initial Spin Filter column. The total plant RNA is then bound to the filter membrane on a second column, washed and finally eluted in 30 – 80 µl of RNasefree water. Rapidly accelerated beads are used to solubilize plant cell walls efficiently, which, in turn, maximizes RNA yields.

Detail Description

1. Homogenization and Lysis of starting material
2. Selective removal of DNA by a first Spin Filter
3. Binding of RNA to a second Spin Filter
4. Washing of bound RNA
5. Final Elution
Specifications
Daten laut MPG  
CE IVDNo
DistributorAnalytik Jena AG
ManufacturerAJ Innuscreen GmbH
Extraction  
A260 : A2801.8 - 2.1
Starting material for extractionUp to 50 mg
Ø YieldIn dependance on the starting material up to 100 µg
Binding capacity> 100 µg RNA
Elution volume30 - 80 µl RNase-free water
Duration of extractionHomogenization and lysis: approx. 15 min, Extraction: approx: 12 min
BasisFilter membrane
HandlingManual processing of Spin Filter Columns
Storage / Stability of extraction kit18 month at room temperature (15 °C to 30 °C)
Packages  
Weight incl. packaging (may differ)300.0 g
Package height (may differ)0.1 m
Width of package (may differ)0.19 m
Length of package (may differ)0.1 m
Applications
  Extraction of Plant RNA
The innuPREP Plant RNA Kit was used to extract total cellular RNA from 50 mg leaf material and compared to the innuSPEED Plant RNA Kit. Subsequent analysis of the quality of the isolated nucleic acids was performed on an Agilent Bioanalyzer. Data indicate total plant RNA with intact ribosomal RNA, free of genomic DNA contamination and with no sign of degradation.

Sample 1: innuSPEED Plant RNA Kit with RIN 9.8; Sample 2: innuPREP Plant RNA Kit with RIN 9,.
Items delivered

Scope of delivery / Kit components

Lysis Tubes P, Lysis Solution, Washing Solutions, RNase-free water, Spin Filter, Receiver Tubes, Elution Tubes,  user Manual

Storage conditions / Stability / Delivery

The innuSPEED Plant RNA Kit will remain stable for at  least 18 months if stored in a dry place at room temperature  (14 °C to 25 °C).