innuSPEED Bacteria/Fungi RNA Kit product photo
innuSPEED Bacteria/Fungi RNA Kit product photo
  • innuSPEED Bacteria/Fungi RNA Kit product photo Front View 2XS

innuSPEED Bacteria/Fungi RNA Kit

Ordernumber: 845-KS-2510010
Manufacturer: AJ Innuscreen GmbH
€ 67.00 plus VAT - free shipping

In Stock

Product Information

Features

  • Extraction of total cellular RNA from fungal spores and gram+ bacteria
  • ƒƒNo DNase I digestion: filter membrane for fast, selective removal of genomic DNA
  • Integrated inactivation of endogenous and exogenous RNases
  • Improved extraction without the use of toxic β-mercaptoethanol
  • ƒƒOptimized for use with homogenizers (such as the SpeedMill)

Productinfo

The innuSPEED Bacteria/Fungi RNA Kit has been designed for rapid homogenization and includes an efficient process for digesting the cell walls of fungal spores and gram+ bacteria in specially developed Lysis Tubes, the process utilizes the SpeedMill (or other homogenizers). The starting sample is then denatured; the unique Lysis Buffer deactivates endogenous and exogenous RNases. Once the genomic DNA has been selectively removed, the RNA is bound to the surface of a Spin Filter membrane, washed and then finally eluted from the membrane. The isolated RNA is immediately available for downstream applications.

Detail Description

1. Homogenize and lyse the starting material; deactivate RNases
2. Perform filtration step to selectively remove genomic DNA
3. Bind the RNA to the Spin Filter
4. Wash the bound RNA
5. Elute
Specifications
Extraction  
A260 : A2801.7 - 2.0
Starting material for extractionGram+ bacteria (max. 1 × 109)
ƒƒFungal spores
Ø YieldDepends on the type and quantity of the starting material
ƒƒUp to 30 µg
Binding capacityColumn binding capacity: > 30 µg RNA
Duration of extractionHomogenization and lysis: approx. 40 minutes
ƒƒPurification: approx. 10 minutes
HandlingNA
Lysis Tubes  
Tube sizeNA
Packages  
Weight incl. packaging (may differ)0.3 kg
Package height (may differ)10.0 cm
Width of package (may differ)19.0 cm
Length of package (may differ)10.0 cm
Applications
RNA was extracted from gram+ bacteria, which are not carrying spores using the innuSPEED Bacteria/Fungi RNA Kit (triple determination). Isolated bacterial RNA was visualized on a denaturing formaldehyde gel.

Lane 1 – 3: Extracted RNA from gram+ bacteria
Items delivered

Scope of delivery / Kit components

Lysis Tubes S, Lysis Solution, Washing Solution, RNase-free water, Spin Filter (blue and purple), Receiver Tubes, Elution Tubes, user manual

Storage conditions / Stability / Delivery

The innuSPEED Bacteria/Fungi DNA Kit will remain stable for at least 12 months if stored in a dry place at room temperature (14 °C to 25 °C).