- Optimum isolation of genomic DNA from gram+ bacteria, yeast and fungal spores
- Includes Lysis Tubes with application-specific beads for highly efficient homogenization with a SpeedMill (or other homogenizers)
- Effective sample disruption for extremely high DNA yields
- High-quality DNA; free of inhibitors
- Product Information
ProductinfoThe innuSPEED Bacteria/Fungi DNA Kit has been specially developed for isolating genomic DNA from gram+ bacteria, fungal spores and yeasts. The extraction protocol combines two steps: rapid homogenization of these hard-to-access starting materials, and efficient digestion of microbial cell walls in specially developed Lysis Tubes (using a SpeedMill or other homogenizer). The material is first broken down mechanically and then subjected to thermal and enzymatic lysis. The DNA is then selectively bound to the surface of a Spin Filter membrane, a step that is followed first by wash steps to remove inhibitors, and then by addition of a low-salt buffer to separate bound DNA from the membrane.
1. Homogenize and lyse the starting material 2. Bind the DNA to the Spin Filter 3. Wash the bound DNA 4. Perform final elution
Extraction A260 : A280 1.7 - 2.0 Starting material for extraction Gram+ bacteria (max. 1 × 109)
Yeasts (max. 1 × 109)
Depends on the type and quantity of the starting material used; up to 30 µg
Binding capacity Column binding capacity: > 30 µg DNA Duration of extraction Homogenization and lysis: approx. 30 – 45 minutes
Purification: approx. 12 minutes
Handling NA Lysis Tubes Tube size NA Packages Weight incl. packaging (may differ) 0.3 kg Package height (may differ) 10.0 cm Width of package (may differ) 19.0 cm Length of package (may differ) 10.0 cm
DNA-Extraktion aus verschiedenen sporenhaltigen Proben und nachfolgende arbiträr geprimte (AP) PCR. Die Visualisierung der PCR-Produkte erfolgte final auf einem TAE-Agarosegel.
Lane 1: DNA-Leiter
Lane 2 – 9: Fragmentmuster einer arbiträr geprimten (AP) PCR unter Verwendung unterschiedlicher Target-DNA
- Items delivered
Scope of delivery / Kit components
Lysis Tubes S, Lysis Solution, Binding Solution, Proteinase K, Washing Solutions, Elution Buffer, Spin Filter (vanilla), Receiver Tubes, Elution Tubes, user manual
Storage conditions / Stability / DeliveryThe innuSPEED Bacteria/Fungi DNA Kit will remain stable for at least 12 months if stored in a dry place at room temperature (14 °C to 25 °C). The recommended storage temperature for lyophilized Proteinase K is 4 °C. Once the Proteinase K has been solubilized, it should be stored in aliquots at –20 °C, because repeated freezing and thawing will significantly reduce its activity.