- Ready-to-use total plant RNA in just 30 minutes after homogenization
- Selection of two integrated Lysis Buffer systems for optimum adaptation to an extremely wide variety of plant species and components
- Selective prefiltration step to prevent DNase digestion
- Optimized extraction chemistry eliminates the need for toxic β-mercaptoethanol
- Product Information
ProductinfoThe chemistry underlying the innuPREP Plant RNA Kit, which has been specially adapted for isolating plant materials (e. g. leaves, caulis, root, blossom), guarantees highly efficient lysis and effectively deactivates endogenous and exogenous RNases. The innuPREP Plant RNA Kit contains two Lysis Buffers in order to process as wide a range as possible of different plant materials, particularly plant components with widely differing characteristics. The Lysis Buffer RL, by comparison, is an universal buffer and the Lysis Buffer PL has been specifically adapted to difficult plants such as roses or potatoes. The total extracted RNA is of excellent quality can be used directly in subsequent applications.
1. Homogenize and lyse the plant material 2. Selectively remove genomic DNA 3. Bind total cellular RNA 4. Wash 5. Elute
Daten laut MPG CE IVD NA Distributor NA Manufacturer NA Extraction A260 : A280 1.8 - 2.1 Starting material for extraction Various types of plant samples (100 mg = max.)
Fresh or frozen plant material
Ø Yield Up to 70 µg
Depends on the type and quantity of the sample
Binding capacity Column binding capacity: approx. 100 µg RNA Elution volume 30 - 80 µl RNase-freies Wasser Duration of extraction Approx. 30 minutes after homogenization Basis Filter membrane Handling NA Storage / Stability of extraction kit 12 month at room temperature (14 °C – 25 °C) Packages Weight incl. packaging (may differ) 1.0 kg Package height (may differ) 15.0 cm Width of package (may differ) 30.0 cm Length of package (may differ) 17.0 cm
A. B. Comparison: RNA extraction from plant materials
The innuPREP Plant RNA Kit was compared to innuSPEED Plant RNA Kit by extraction of total cellular RNA from a 50 mg leaf material. The subsequent analysis of the quality of the isolated nucleic acid was performed on an Agilent Bioanalyzer. Data indicate total plant RNA with intact ribosomal RNA, free of genomic DNA contamination and with no sign of degradation.
innuSPEED Plant RNA Kit with RIN 9.8
innuPREP Plant RNA Kit with RIN 9.5
- Items delivered
Scope of delivery / Kit components
Lysis Solution, Washing Solutions, RNase-free water, Spin Filter (blue and purple), Receiver Tubes, Elution Tubes, user manual
Storage conditions / Stability / Delivery
The innuPREP Plant RNA Kit will remain stable for at least 12 months if stored in a dry place at room temperature (14 °C to 25 °C).