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  4. innuDRY Standard PCR MasterMix
innuDRY Standard PCR MasterMix product photo
innuDRY Standard PCR MasterMix product photo
  • innuDRY Standard PCR MasterMix product photo Front View 2XS

innuDRY Standard PCR MasterMix

Lyophilized Master Mix for PCR

Ordernumber: 845-AS-2100200
Manufacturer: AJ Innuscreen GmbH
€ 122.00 plus VAT - free shipping

In Stock

Product Information

Features

  • Lyophilized Master Mix for PCR
  • Easy handling thanks to 2times concentration
  • Environmentally beneficial: stable and delivered at room temperature
  • Ready-to-use including hot start Taq DNA Polymerase, dNTP’s and optimal buffer conditions
  • Ideal results especially for routine PCR applications
  • Safe and secure
  • Minimizing of necessary hands on steps

Productinfo

Especially in daily routine tasks easy and secure procedures, which guarantee a high reproducibility of final results are very important. In addition stable reagents with a reliable performance are fundamental as well. Der innuDRY Standard PCR MasterMix combines all requirements. The lyophilized mix is long-term storage stable and can be dissolved by the included Resuspension Buffer at any time. After addition of template and specific primers the PCR reaction just needs to be filled up to final volume by using molecular biological water and is ready-to-use for subsequent amplification. The use of innuDRY Standard PCR MasterMixe can be easily combined with most commercially available thermal cyclers. Thereby quality as well as yield of amplified PCR products is excellent.

Specifications
Reagents  
CompatibilityTProfessional series and TAdvanced series, FlexCycler² and SpeedCycler² SP, etc.
Concentration2x
Storage / Stability of reagents12 month at +4 °C
Additional InformationComposition: innuTaq HOT-A DNA Polymerase, dNTP’s, Enhancer and MgCl2
ConditionLyophilized
Spitzen  
CompatibilityTProfessional series and TAdvanced series, FlexCycler² and SpeedCycler² SP, etc.
Packages  
Weight incl. packaging (may differ)0.0 g
Package height (may differ)0.0 m
Width of package (may differ)0.0 m
Length of package (may differ)0.0 m
Applications
A.
B. 
Comparison of innuMIX and innuDRY: amplification of a 1 kb Lambda DNA fragment
A 1 kb fragment (Lambda DNA) was amplified in 6times determination by using the innuDRY Standard PCR MasterMix in comparison to the innuMIX Standard PCR MasterMix. An initial denaturation step of 2 min was followed by 30 cycles with 15 sec denaturation, 15 sec annealing and 30 sec elongation. Finally an Elongation step of further 60 s was carried out. 
C. 
D.  
A.
Lane 1,8: DNA ladder
Lane 2 – 7: 1 kb fragment using innuMIX Standard PCR MasterMix and TAdvanced
B.
Lane 1,8: DNA ladder
Lane 2 – 7: 1 kb fragment using innuDRY Standard PCR MasterMix and TAdvanced
C.
Lane 1,8: DNA ladder
Lane 2 – 7: 1 kb fragment using innuMIX Standard PCR MasterMix and SpeedCycler² SP
D.
Lane 1,8: DNA ladder
Lane 2 – 7: 1 kb fragment using innuDRY Standard PCR MasterMix and SpeedCycler² SP
       
A.
B.
Comparison of innuMIX and innuDRY: amplification of a 210 bp fragment of human genomic DNA
The innuDRY Standard PCR MasterMix was compared to the innuMIX Standard PCR MasterMix by amplification of a 210 bp b-Globin fragment (human gDNA). Each sample was determined 6times using TAdvanced and SpeedCycler² SP (initial denaturation 2 min, 30x 15 sec denaturation, 15 sec annealing, 30 sec elongation, final elongation 60 sec).
C. D.
A.
Lane 1,8: DNA ladder
Lane 2 – 7: 210 bp b-Globin using innuMIX Standard PCR MasterMix and TAdvanced
B.
Lane 1,8: DNA ladder
Lane 2 – 7: 210 bp b-Globin using innuDRY Standard PCR MasterMix and TAdvanced
C.
Lane 1,8: DNA ladder
Lane 2 – 7: 210 bp b-Globin using innuMIX Standard PCR MasterMix and SpeedCycler² SP
D.
Lane 1,8: DNA ladder
Lane 2 – 7: 210 bp b-Globin using innuDRY Standard PCR MasterMix and SpeedCycler² SP
Items delivered

Scope of delivery / Kit components

2times concentrated master mix (lyophilized); Resuspension Buffer

Storage conditions / Stability / Delivery

The innuDRY Standard PCR MasterMix is delivered at room temperature (14 °C to 25 °C). The recommended storage temperature for lyophilized Master Mix is 4 °C. Once the Mix has been solubilized, it should be stored at –20 °C. The mix is stable for at least 12 month under these conditions.