1. Analytik Jena
  2. Reagents
  3. Real-time PCR: master mixes
  4. innuDRY qPCR MasterMix Probe
innuDRY qPCR MasterMix Probe product photo
innuDRY qPCR MasterMix Probe product photo
  • innuDRY qPCR MasterMix Probe product photo Front View 2XS

innuDRY qPCR MasterMix Probe

Lyophilized Master Mix for qPCR

Ordernumber: 845-AS-1900100
Manufacturer: AJ Innuscreen GmbH
€ 102.00 plus VAT - free shipping

In Stock

Product Information

Features

  • Lyophilized Master Mix for real-time PCR
  • Ideal for usage with different probe detection technologies (incl. Taqman® or Rehybridization Probes)
  • Environment-friendly delivery at room temperature
  • 2times concentrated for easy and safe handling
  • Compatible to most real-time PCR thermal cyclers
  • Including high-quality dNTP’s and a Taq DNA Polymerase with Hot Start function
  • High storage stable at +4 °C

Productinfo

The innuDRY qPCR MasterMix Probe is a novel, storage stable real-time PCR Master Mix for fluorescence detection using different probe technologies (e.g. hydrolysis, FRET or rehybridization probes, and many more). The mix contains all components for a successful real-time PCR. Besides high-quality dNTP’s and a perfect combination of qPCR enhancers, the hot start polymerase avoids unspecific side products selectively. Additionally the lyophilized 2times formulation offers high storage stability at +4 °C and an environmentally friendly delivery at room temperature.

After dissolving by using the Resuspension Buffer only primer, probes and the template need to be add to the master mix and filled up to final volume by using molecular biological water. The established handling makes the daily use easy and secure. Real-time results are highly reproducible and ideal with regards to efficiency and slope.
Specifications
Reagents  
CompatibilityTProfessional-Serie and TAdvanced-Serie, FlexCycler² and SpeedCycler² SP, etc.
Concentration2x
Storage / Stability of reagents12 month at +4 °C
Additional InformationComposition: innuTaq HOT-A DNA Polymerase, dNTP’s, Enhancer and MgCl2
ConditionLyophilized
Spitzen  
CompatibilityTProfessional-Serie and TAdvanced-Serie, FlexCycler² and SpeedCycler² SP, etc.
Packages  
Weight incl. packaging (may differ)0.0 g
Package height (may differ)0.0 m
Width of package (may differ)0.0 m
Length of package (may differ)0.0 m
Applications
A.
 B.

Real-Time amplification based on usage of Tqman® probes
The SRY-Gen of human genomic DNA was amplified by using Taqman® probes and qTOWER 2 real-time thermal cycler. For ideal comparison of innuDRY qPCR MasterMix Probe and innuMIX qPCR MasterMix Probe, identical probes were labelled with different fluorescent dyes (FAM, YakimaYellow, ROX and Cy5) and used in a singleplex run.

Plots Rot: innuDRY qPCR Master Mix Probe jeweils in 3fach Bestimmung
Plots Schwarz: innuMIX qPCR Master Mix Probe jeweils in 3fach Bestimmung

 

Ct-Werte

A. FAM probe

B. YakimaYellow probe

C. ROX probe

D. Cy5 probe

innuDRY qPCR Master Mix Probe

26.35

26.19

25.29

25.23

26.18

26.15

25.16

25.25

26.02

26.22

25.26

25.38

innuMIX qPCR Master Mix Probe

26.44

26.02

25.15

25.25

26.45

25.90

25.33

25.22

26.43

26.02

25.29

25.20

 

 C.
D.
Items delivered

Scope of delivery / Kit components

2times concentrated master mix (lyophilized); Resuspension Buffer

Storage conditions / Stability / Delivery

The innuDRY MasterMix Probe is delieverd at room temperature (14 °C to 25 °C). The recommended storage temperature for lyophilized Master Mix is 4 °C. Once the Mix has been solubilized, it should be stored at –20 °C. The mix is stable for at least 12 month under these conditions.